To collect and standardize the ever-growing volume of omics data (DNA or RNA sequences, metabolomics, etc.) produced by Great Lakes researchers, we built the Great Lakes Atlas of Multi-omics Research (GLAMR), a publicly-accessible website and database supported via the NOAA ‘Omics program. To populate GLAMR, we collected Great Lakes metagenomic, metatranscriptomic, and amplicon sequencing datasets “including many of our own” from public repositories while simultaneously collecting and standardizing all available sample metadata, including associated environmental measurements. The GLAMR website, greatlakesomics.org, allows users to explore, query, and download datasets processed through standardized bioinformatics pipelines, and provides links to the originating repositories. GLAMR currently houses nearly 2,500 samples and we expect this number to grow rapidly as researchers increasingly rely on omics methods. By bringing together diverse datasets spanning 15 years from across the Great Lakes, GLAMR provides researchers and other Great Lakes stakeholders a powerful but accessible tool for understanding this critical freshwater ecosystem.